aeruginosa flowing through microfluidic channels made from PDMS formed streamer structures resembling biofilm bridges, causing clogging. used micropatterened poly(dimethyl siloxane) (PDMS) to form 3D nanostring of microcolonies of Staphyloccocus aureus. īridge or string-like structures of bacteria colonies were reported in biofilm studies previously. aeruginosa are able to achieve adaptive antibiotic resistance by living together as biofilms. Except for its high level of intrinsic resistance, Gram-negative bacteria such as P. Various mechanisms including active efflux of antibiotics, cell wall barrier, enzymatic inactivation of drugs, and/or antibiotic target changes/protection contribute to the antibiotic resistance of Gram-negative bacteria. aeruginosa is a major cause of chronic infections strongly involved in cystic fibrosis patients and immunocompromised individuals. are widely spread in natural and artificial environments. Gram-negative bacteria such as Acinetobacter spp., Pseudomonas aeruginosa ( P.
Thus, bacterial bridges can be used to study formation, spreading, and development of bacterial colonies, and communication within and between isolated biofilms. It is demonstrated that bacterial bridges connecting bacteria colonies act as bio-microfluidic channels and can transport liquids, nutrients, and antibacterial substances between neighboring bacteria clusters. The geometry and distribution of bridges can be controlled using predesigned superhydrophobic–hydrophilic patterns. It is demonstrated that the bacteria attached to hydrophilic areas and bacteria precipitated on lubricant infused borders both contribute to the formation of bacterial bridges. Here patterned lubricant-infused surfaces (pLIS) are utilized to fabricate connective structures named “bacterial bridges” between bacterial colonies of Pseudomonas aeruginosa by a simple dewetting method. Spatial control of bacteria and biofilms on surfaces is necessary to understand the biofilm formation and the social interactions between bacterial communities, which could provide useful hints to study the biofilm-involved diseases.
0 kommentar(er)
